Kasprzak K S, Sunderman F W
Res Commun Chem Pathol Pharmacol. 1977 Jan;16(1):95-108.
To elucidate the initial steps of carcinogenesis by nickel subsulfide, the mechanisms of dissolution of alpha-Ni3S2 were studied by incubating alpha-Ni3S2 dust in (a) water, (b) rat serum, and (c) rat serum ultrafiltrate. The sediments that remained following incubation of alpha-Ni3S2 in the 3 media for 2 weeks were examined by X-ray diffractometry. The rates of solubilization of 63Ni from alpha-63Ni3S2 during incubation in the 3 media were measured by liquid scintillation counting. These studies showed that solubilization of Ni(II) from alpha-Ni3S2 in rat serum requires the presence of O2 and involves three reactions: [I] 2 alpha-Ni3S2 + O2 + 2H2O leads to 4 beta-NiS + 2Ni(OH)2; [ii] beta-NiS + 202 leads to Ni2+ + SO4(2-); and [III] Ni2+ & Ni(OH)2 + serum ligands [albumin & amino acids] leads to soluble Ni(II)-complexes. The rate of solubilization of 63Ni from alpha-63Ni3S2 was initially more rapid in rat serum than in serum ultrafiltrate and much more rapid than in water. After 2 days of incubation the rates of dissolution of alpha-63Ni3S2 in the 3 media became progressively retarded, probably owing to deposition of insoluble surface coatings of beta-63Nis and 63Ni(OH)2 on the alpha-63Ni3S2 particles.
为阐明硫化镍致癌的初始步骤，通过将α-Ni3S2粉尘分别置于（a）水、（b）大鼠血清和（c）大鼠血清超滤液中孵育，研究了α-Ni3S2的溶解机制。通过X射线衍射法检测了α-Ni3S2在这三种介质中孵育2周后剩余的沉淀物。通过液体闪烁计数法测定了α-63Ni3S2在这三种介质中孵育期间63Ni的溶解速率。这些研究表明，α-Ni3S2在大鼠血清中溶解出Ni（II）需要O2的存在，且涉及三个反应：[I] 2α-Ni3S2 + O2 + 2H2O → 4β-NiS + 2Ni(OH)2；[ii] β-NiS + 2O2 → Ni2+ + SO4(2-)；以及[III] Ni2+ & Ni(OH)2 + 血清配体[白蛋白和氨基酸] → 可溶性Ni（II）络合物。α-63Ni3S2中63Ni的溶解速率最初在大鼠血清中比在血清超滤液中更快，且比在水中快得多。孵育2天后，α-63Ni3S2在这三种介质中的溶解速率逐渐减慢，这可能是由于β-63Nis和63Ni(OH)2不溶性表面涂层沉积在α-63Ni3S2颗粒上所致。
