Aoyagi Y, Ikenaka T, Ichida F
Cancer Res. 1977 Oct;37(10):3663-7.
Human alpha-fetoproteins were purified from umbilical cord serum and from ascites fluid of a patient with hepatoma by affinity chromatography, and their chemical compositions and terminal sequences were compared. The amino acid compositions of these alpha-fetoproteins were similar and in good agreement with the values reported by other investigators. The COOH-terminal 5-amino acid sequence determined by carboxypeptidase digestion and the NH2-terminal 20-amino acid sequence determined by an automated sequence analyzer revealed that both alpha-fetoproteins had the same terminal sequences of amino acids. The sequence analysis showed that a part of each of the proteins lacked its NH2-terminal residues for one or three amino acids. A small difference in the carbohydrate composition of each alpha-fetoprotein was observed. It was concluded that alpha-fetoproteins from fetal serum and from ascites fluid of a patient with hepatoma had very similar structures.
通过亲和层析从脐带血清和一名肝癌患者的腹水中纯化出人甲胎蛋白，并比较了它们的化学组成和末端序列。这些甲胎蛋白的氨基酸组成相似，与其他研究者报道的值高度一致。通过羧肽酶消化确定的COOH末端5个氨基酸序列以及通过自动序列分析仪确定的NH2末端20个氨基酸序列显示，两种甲胎蛋白具有相同的氨基酸末端序列。序列分析表明，每种蛋白质的一部分缺少1个或3个氨基酸的NH2末端残基。观察到每种甲胎蛋白的碳水化合物组成存在微小差异。得出的结论是，来自胎儿血清和肝癌患者腹水的甲胎蛋白具有非常相似的结构。
