Caraux J, Weigle W O
Cell Immunol. 1983 May;78(1):23-32. doi: 10.1016/0008-8749(83)90256-3.
The present report demonstrates that cells expressing idiotypic determinants on their surfaces can be specifically destroyed by anti-idiotypic antibodies acting as inducers of antibody-dependent cell-mediated cytotoxicity. Lysis of surface-idiotype-positive hybridoma cells was effected by mouse spleen cells, mouse peritoneal cells, or human blood mononuclear cells, in the presence of anti-idiotypic antibodies. In order for lysis to occur, the simultaneous presence of Fc receptor-bearing cytotoxic cells and anti-idiotypic antibodies was required. Specificity of lysis was conferred by the anti-idiotypic antibody. Hybridoma cells expressing a different idiotype could not be lysed. Anti-idiotypic antibody-dependent cell-mediated cytotoxicity against 51Cr-labeled cells expressing a particular idiotype could be induced by the specific anti-idiotypic antibodies and inhibited by unlabeled idiotype-positive cells or by idiotype in a soluble form. Cytotoxicity could not be inhibited by the presence of bystander cells or monoclonal antibodies expressing a different idiotype. It is proposed that ADCC lysis of idiotype-positive cells has important implications for understanding the regulation of immune responses by an idiotype-anti-idiotype network. This cytolytic pathway provides an extremely specific and effective mode of control of idiotype-positive cells by anti-idiotype antibody. Previous observations that an intact Fc fragment was necessary for anti-idiotype antibodies to exert suppressive effects in vivo further support this hypothesis.
本报告表明，表面表达独特型决定簇的细胞可被作为抗体依赖性细胞介导的细胞毒性诱导剂的抗独特型抗体特异性破坏。在抗独特型抗体存在的情况下，小鼠脾细胞、小鼠腹腔细胞或人血单核细胞可对表面独特型阳性杂交瘤细胞进行裂解。为了发生裂解，需要同时存在带有Fc受体的细胞毒性细胞和抗独特型抗体。裂解的特异性由抗独特型抗体赋予。表达不同独特型的杂交瘤细胞不能被裂解。特异性抗独特型抗体可诱导针对表达特定独特型的51Cr标记细胞的抗独特型抗体依赖性细胞介导的细胞毒性，未标记的独特型阳性细胞或可溶性形式的独特型可抑制该毒性。旁观者细胞或表达不同独特型的单克隆抗体的存在不能抑制细胞毒性。有人提出，独特型阳性细胞的ADCC裂解对于理解独特型-抗独特型网络对免疫反应的调节具有重要意义。这种细胞溶解途径提供了一种由抗独特型抗体极其特异性和有效地控制独特型阳性细胞的方式。以前的观察结果表明，完整的Fc片段是抗独特型抗体在体内发挥抑制作用所必需的，这进一步支持了这一假说。
