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体内用诱变致癌杂环胺对核酸进行修饰。从注射了3-氨基-1-甲基-5H-吡啶并[4,3-b]吲哚和2-氨基-6-甲基二吡啶并[1,2-a:3',2'-d]咪唑的大鼠中提取的DNA中修饰碱基的鉴定。

Modification of nucleic acids with muta-carcinogenic heteroaromatic amines in vivo. Identification of modified bases in DNA extracted from rats injected with 3-amino-1-methyl-5H-pyrido[4,3-b]indole and 2-amino-6-methyldipyrido[1,2-a3:3',2'-d]imidazole.

作者信息

Hashimoto Y, Shudo K, Okamoto T

出版信息

Mutat Res. 1982 Aug;105(1-2):9-13. doi: 10.1016/0165-7992(82)90200-7.

DOI:10.1016/0165-7992(82)90200-7
PMID:7121483
Abstract

Potent muta-carcinogens isolated from amino-acid pyrolysates, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and 2-amino-1-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1), were injected intraperitoneally into Male Wistar rats. DNA and rRNA modified with these muta-carcinogens were then extracted from the livers of the rats, and modified nucleic-acid bases were isolated and analysed by high-performance liquid chromatography (HPLC). These modified nucleic-acid bases were identified as 3-(C8-guanyl)amino-1-methyl-5H-pyrido[4,3-b]indole (Gua-Trp-P-2) and 2-(C8-guanyl)amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Gua-Glu-P-1) by comparison of their retention times on HPLC and UV spectra with those of authentic compounds.

摘要

从氨基酸热解产物中分离出的强效诱变致癌物3-氨基-1-甲基-5H-吡啶并[4,3-b]吲哚（Trp-P-2）和2-氨基-1-甲基二吡啶并[1,2-a:3',2'-d]咪唑（Glu-P-1），经腹腔注射到雄性Wistar大鼠体内。然后从大鼠肝脏中提取用这些诱变致癌物修饰的DNA和rRNA，并通过高效液相色谱法（HPLC）分离和分析修饰的核酸碱基。通过比较它们在HPLC上的保留时间和紫外光谱与标准化合物的保留时间和紫外光谱，将这些修饰的核酸碱基鉴定为3-（C8-鸟嘌呤基）氨基-1-甲基-5H-吡啶并[4,3-b]吲哚（Gua-Trp-P-2）和2-（C8-鸟嘌呤基）氨基-6-甲基二吡啶并[1,2-a:3',2'-d]咪唑（Gua-Glu-P-1）。

相似文献

1
Modification of nucleic acids with muta-carcinogenic heteroaromatic amines in vivo. Identification of modified bases in DNA extracted from rats injected with 3-amino-1-methyl-5H-pyrido[4,3-b]indole and 2-amino-6-methyldipyrido[1,2-a3:3',2'-d]imidazole.体内用诱变致癌杂环胺对核酸进行修饰。从注射了3-氨基-1-甲基-5H-吡啶并[4,3-b]吲哚和2-氨基-6-甲基二吡啶并[1,2-a:3',2'-d]咪唑的大鼠中提取的DNA中修饰碱基的鉴定。
Mutat Res. 1982 Aug;105(1-2):9-13. doi: 10.1016/0165-7992(82)90200-7.
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Mutat Res. 1983 Jul;118(1-2):91-102. doi: 10.1016/0165-1218(83)90119-2.

引用本文的文献

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The rat urinary bladder as a new target of heterocyclic amine carcinogenicity: tumor induction by 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate.大鼠膀胱作为杂环胺致癌作用的新靶点：3-氨基-1-甲基-5H-吡啶并[4,3-b]吲哚乙酸诱导肿瘤
Jpn J Cancer Res. 1993 Aug;84(8):852-8. doi: 10.1111/j.1349-7006.1993.tb02057.x.
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用诱变致癌物对DNA进行化学修饰。I. 3-氨基-1-甲基-5H-吡啶并[4,3-b]吲哚和2-氨基-6-甲基二吡啶并[1,2-a:3',2'-d]咪唑：DNA加合物的代谢活化与结构
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A current genotoxicity database for heterocyclic thermic food mutagens. I. Genetically relevant endpoints.杂环热食诱变剂的当前遗传毒性数据库。I. 与遗传相关的终点。
Environ Health Perspect. 1986 Aug;67:93-103. doi: 10.1289/ehp.866793.