Liss A, Cole R M
Rev Infect Dis. 1982 May-Jun;4 Suppl:S115-9. doi: 10.1093/clinids/4.supplement_1.s115.
Virus-like particles of three morphologic groups have been detected by electron microscopy in 90% of spiroplasma strains. In this study, virus-like particles of group 1 (SpV1)--unenveloped rods 230-280 nm by 10-15 nm--were found in 575 of spiroplasmas. SpV1 occurred spontaneously in large numbers in cultures of strains B (corn stunt), G1 (tulip tree flower), BC3 (honeybee), and KC3 (honeybee), respectively; the viruses were isolated and assayed as plaque-forming units, with several of the same strains used as indicators. No virus plaqued on its strain of origin, and all except one (SpV1/BC3) plaqued best on strain BC3. Host range and yields of progeny virus differed. All isolates were identical in morphology, size, and buoyant densities; contained DNA; formed turbid 1.5 to 5.0-mm plaques; produced nonlytic infections and similar one-step growth curves; were inactivated by a single antiserum; had similar kinetics of adsorption and heat inactivation; and demonstrated similar patterns of resistance and sensitivity to a variety of physical and chemical agents, as well as an inability to infect resistant host mutants. SPV1 isolates from diverse host strains apparently share many physicochemical and biological properties, but are not identical in all biological respects.
通过电子显微镜在90%的螺旋体菌株中检测到了三种形态学类型的病毒样颗粒。在本研究中，在575株螺旋体中发现了第1组病毒样颗粒（SpV1）——无包膜的杆状颗粒，长230 - 280纳米，宽10 - 15纳米。SpV1分别在菌株B（玉米矮化病）、G1（鹅掌楸花）、BC3（蜜蜂）和KC3（蜜蜂）的培养物中大量自发出现；这些病毒被分离出来并以噬斑形成单位进行测定，使用了几种相同的菌株作为指示菌。没有病毒在其起源菌株上形成噬斑，除了一种（SpV1/BC3）外，所有病毒在BC3菌株上形成噬斑的效果最好。子代病毒的宿主范围和产量有所不同。所有分离株在形态、大小和浮力密度方面均相同；含有DNA；形成1.5至5.0毫米的浑浊噬斑；产生非裂解性感染并具有相似的一步生长曲线；被单一抗血清灭活；具有相似的吸附动力学和热灭活特性；对多种物理和化学试剂表现出相似的抗性和敏感性模式，并且无法感染抗性宿主突变体。来自不同宿主菌株的SPV1分离株显然具有许多物理化学和生物学特性，但在所有生物学方面并不完全相同。
