Kobayashi M, Mukai N, Solish S P, Pomeroy M E
Acta Neuropathol. 1982;57(2-3):203-8. doi: 10.1007/BF00685390.
A new animal model of retinoblastoma was developed in newborn inbred CDF rats by intravitreous inoculation of retinal tumor cells (5 X 10(4)/5 microliter) derived from the cultured tumor cell line EXP-5. The retinal tumor from which the cell line originated was induced by a single intravitreous inoculation of human adenovirus serotype 12 (5 microliter of 10(8) TCID 50/0.1 ml) in syngeneic rats. Within 1 month after intravitreous inoculation of EXP-5 cells, a clinically recognizable ocular tumor was obtained in all 39 rats. Ad 12-specific T-antigens were demonstrated in the cultured tumor cells using immunofluorescent techniques. Morphologically these tumor cells closely resembled retinoblastoma, with poorly differentiated intracytoplasmic organelles, solitary cilia with a 9 + 0 tubule pattern, and abnormal nuclear membrane associated with a set of basal bodies. The significance of this highly manipulable retinal tumor cell line is the capability of providing a full-fledged intravitreous tumor in 1-month-old CDF rats, whose actual life span is known to be 42 months. Transplantable retinal tumors described to date are reviewed briefly and compared with the presently reported cell line.
通过向新生近交系CDF大鼠玻璃体内接种源自培养的肿瘤细胞系EXP-5的视网膜肿瘤细胞（5×10⁴/5微升），建立了一种新的视网膜母细胞瘤动物模型。该细胞系所源自的视网膜肿瘤是通过向同基因大鼠玻璃体内单次接种1型人腺病毒（5微升，10⁸半数组织培养感染剂量50/0.1毫升）诱导产生的。在向玻璃体内接种EXP-5细胞后的1个月内，所有39只大鼠均出现了临床上可识别的眼内肿瘤。使用免疫荧光技术在培养的肿瘤细胞中证实了腺病毒12特异性T抗原。从形态学上看，这些肿瘤细胞与视网膜母细胞瘤极为相似，胞质内细胞器分化不良，有9 + 0微管模式的单个纤毛，以及与一组基体相关的异常核膜。这种高度可操作的视网膜肿瘤细胞系的意义在于，它能够在1月龄的CDF大鼠体内形成成熟的玻璃体内肿瘤，已知其实际寿命为42个月。本文简要回顾了迄今为止所描述的可移植视网膜肿瘤，并将其与目前报道的细胞系进行了比较。
