Kato Y, Matsumoto H, Kobayashi M, Okada Y, Kawaoi A
Second Department of Pathology, Yamanashi Medical College, Japan.
Acta Pathol Jpn. 1991 Nov;41(11):811-7. doi: 10.1111/j.1440-1827.1991.tb01623.x.
To elucidate the pathogenesis of human retinoblastoma, we investigated the genomic expression in retinal tumors induced by human adenovirus type 12 in rats, using various DNA probes. Seven rats received a single intraocular inoculation of concentrated virus fluid within 24 hours after birth. Intravitreous tumors were induced in two out of seven animals (28.5%) within 30 to 64 days after the inoculation. A remarkably uniform histologic feature, i.e., neuroblastic cells in association with Homer-Wright pseudorosettes, was present in all cases. The adenovirus-related oncoprotein gene E1A and human retinoblastoma susceptibility gene were detected in the tumors by Southern blot hybridization. In situ hybridization analysis demonstrated expression of adenovirus type 12 E1A gene in the inner granular layer of the retina. It was suggested that integration of adenovirus type 12 E1A fragment with the host genome and expression of the gene were required for induction of this tumor.
为阐明人类视网膜母细胞瘤的发病机制，我们使用多种DNA探针研究了12型人腺病毒在大鼠体内诱导产生的视网膜肿瘤的基因组表达。7只大鼠在出生后24小时内接受了一次眼内注射浓缩病毒液。接种后30至64天内，7只动物中有2只（28.5%）诱发了玻璃体内肿瘤。所有病例均呈现出显著一致的组织学特征，即神经母细胞伴霍纳-赖特假菊形团。通过Southern印迹杂交在肿瘤中检测到腺病毒相关癌蛋白基因E1A和人类视网膜母细胞瘤易感基因。原位杂交分析表明，12型腺病毒E1A基因在视网膜内颗粒层表达。提示12型腺病毒E1A片段与宿主基因组整合并表达该基因是诱导此肿瘤所必需的。
