Fuentes J, Miró J, Riera J
Andrologia. 1982 Jul-Aug;14(4):322-7. doi: 10.1111/j.1439-0272.1982.tb02270.x.
Zinc concentration in seminal plasma is one of the best biochemical parameters with which to assess prostatic function in the study of male infertility. Nevertheless, this parameter is not determined in many laboratories because of technical difficulties. In this paper a simple colorimetric method for seminal plasma zinc assay is presented. This assay is performed with only one reagent solution making deproteinization and extraction with organic solvents unnecessary. The method is based on the reaction between 1-(2-pyridylazo)-2-naphthol and zinc which yields a coloured complex, that is soluble in the reagent solution when dodecyl hydrogen sulfate sodium salt is present. The solubilized complex has an absorbance peak at 560 nm. The accuracy of this method is proved by statistical comparison with atomic absorption spectrophotometry. Furthermore, the method has a good precision and is free of interference.
在男性不育症研究中，精浆中的锌浓度是评估前列腺功能的最佳生化参数之一。然而，由于技术困难，许多实验室并未测定该参数。本文介绍了一种用于精浆锌测定的简单比色法。该测定仅需一种试剂溶液，无需进行脱蛋白和用有机溶剂萃取。该方法基于1-（2-吡啶偶氮）-2-萘酚与锌之间的反应，生成一种有色络合物，当存在十二烷基硫酸氢钠时，该络合物可溶于试剂溶液。溶解的络合物在560nm处有一个吸收峰。通过与原子吸收分光光度法的统计比较，证明了该方法的准确性。此外，该方法具有良好的精密度且无干扰。
