Heinen W, Lauwers A M, Mulders J W
Antonie Van Leeuwenhoek. 1982;48(3):265-72. doi: 10.1007/BF00400386.
A sample from a hot spring on the northern island of New Zealand contained five different thermophilic bacterial strains. One strain with peculiar properties, i.e. the formation of dark yellow colonies at 30 degrees C as well as at 70 degrees C, was further characterized. It was found to be a gram-positive, facultatively aerobic, motile Bacillus species, with terminal endospores. According to the physiologic properties the strain closely resembled B. coagulans. However, two typical characteristics were contradictory to this conclusion, namely the intense yellow pigmentation of the colonies and the range of growth temperature. The latter was found to reach from 40 to 70 degrees C, with an optimum at 60 degrees C under aerobic and at 65 degrees C under anaerobic conditions. Growth at moderate temperatures was slower than at 60 degrees C, but the final cell yields were almost equal. The strain can therefore be considered as facultatively thermophilic. The pigment, which was found to be located in the cytoplasmic membrane, was spectroscopically identified as a carotenoid. Because the characteristics of this strain did not correspond with any of the Bacillus species described thus far, we concluded, that we had isolated a novel strain, for which the name Bacillus flavothermus is proposed.
从新西兰北岛的一个温泉中采集的样本包含五种不同的嗜热细菌菌株。对其中一种具有特殊性质的菌株进行了进一步鉴定，即在30摄氏度和70摄氏度时均能形成深黄色菌落。结果发现它是一种革兰氏阳性、兼性需氧、能运动的芽孢杆菌属物种，具有末端芽孢。根据生理特性，该菌株与凝结芽孢杆菌非常相似。然而，有两个典型特征与这一结论相矛盾，即菌落强烈的黄色色素沉着和生长温度范围。发现其生长温度范围为40至70摄氏度，有氧条件下最适温度为60摄氏度，厌氧条件下为65摄氏度。在中等温度下的生长比在60摄氏度时慢，但最终细胞产量几乎相等。因此，该菌株可被视为兼性嗜热菌。发现该色素位于细胞质膜中，经光谱鉴定为类胡萝卜素。由于该菌株的特征与迄今描述的任何芽孢杆菌属物种都不相符，我们得出结论，我们分离出了一个新菌株，并提议将其命名为嗜热黄芽孢杆菌。
