Nuttall P A, Harrap K A
Arch Virol. 1982;73(1):1-13. doi: 10.1007/BF01341722.
A virus was isolated from 2 day-old mice inoculated with homogenates of either the lungs or blood of 2 different shearwaters affected by puffinosis. Examination of infected suckling mouse brain and infected NCTC-1469 (mouse liver) cell cultures, by electron microscopy, revealed virus particles and inclusion bodies characteristic of a coronavirus. Neutralization, complement fixation and fluorescent antibody tests showed that the virus was related to mouse hepatitis virus. The virus was not isolated from 445 control, uninfected mice. Neutralising antibodies were not detected in 39 sera from diseased shearwaters and 2 sera from apparently healthy birds. Two shearwaters inoculated with the virus did not develop clinical signs of infection. The question of whether the virus was isolated from shearwaters or from laboratory mice is discussed.
从接种了受海鹦病影响的2种不同剪水鹱的肺或血液匀浆的2日龄小鼠中分离出一种病毒。通过电子显微镜检查受感染的乳鼠脑和受感染的NCTC - 1469（小鼠肝脏）细胞培养物，发现了冠状病毒特有的病毒颗粒和包涵体。中和试验、补体结合试验和荧光抗体试验表明该病毒与小鼠肝炎病毒有关。未从445只未感染的对照小鼠中分离出该病毒。在39份患病剪水鹱的血清和2份明显健康鸟类的血清中未检测到中和抗体。接种该病毒的2只剪水鹱未出现感染的临床症状。文中讨论了该病毒是从剪水鹱还是从实验室小鼠中分离出来的问题。
