Wilschut J, Holsappel M, Jansen R
Biochim Biophys Acta. 1982 Sep 9;690(2):297-301. doi: 10.1016/0005-2736(82)90334-0.
The kinetics of Ca2+-induced fusion of large (0.1 micrometer) unilamellar cardiolipin/phosphatidylcholine (1:1) vesicles have been investigated by continuous monitoring of the mixing of the aqueous vesicle contents. In parallel, release of vesicle contents to the external medium has been followed. Initial fusion of the vesicles is non-leaky, release of vesicle contents to the external medium have been followed. Initial fusion of the vesicles is non-leaky, release of vesicle contents being largely a secondary phenomenon. The minimal Ca2+ concentration required for fusion in this system is approx. 9 mM. At higher Ca2+ concentrations fusion is extremely fast, occurring on the time scale of seconds.
通过连续监测水泡内水相内容物的混合情况，研究了Ca2+诱导的大尺寸（0.1微米）单层心磷脂/磷脂酰胆碱（1:1）囊泡融合的动力学。同时，跟踪了囊泡内容物向外部介质的释放情况。囊泡的初始融合是无泄漏的，随后跟踪了囊泡内容物向外部介质的释放。囊泡的初始融合是无泄漏的，囊泡内容物的释放主要是一种次要现象。该系统中融合所需的最低Ca2+浓度约为9 mM。在较高的Ca2+浓度下，融合极其迅速，发生在秒级时间尺度上。
