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[质子在与膜相邻层中的结合-分离反应中双层脂质膜上电势的产生]

[Generation of potential on bilayer lipid membrane in proton combination-separation reactions in the layers adjacent to the membranes].

作者信息

Antonenko Iu N, Iaguzhinskiĭ L S

出版信息

Biofizika. 1982 Jul-Aug;27(4):635-40.

PMID:7126660
Abstract

Addition of uncoupler at the background of concentration gradient of the salts of weak acids and bases (sodium acetate and ammonium chloride) brought about formation of the positive potential on the BLM part with high sodium acetate concentration and of the negative potential on the BLM part with high ammonium chloride concentration. It was shown that the potential formation was conditioned by the initiation of pH gradient in unstirred layers. The effects observed are described by means of the earlier developed [7] model of the transfer of weak acids and bases through BLM. The results obtained show that with the existence of gradients of weak acids and bases potentials may appear on the membrane, biological ones included, in the presence of protonophor.

摘要

在弱酸和弱碱盐(醋酸钠和氯化铵)浓度梯度的背景下添加解偶联剂,会在醋酸钠浓度高的BLM部分形成正电位,在氯化铵浓度高的BLM部分形成负电位。结果表明,电位的形成是由未搅拌层中pH梯度的启动所决定的。通过早期开发的[7]弱酸和弱碱通过BLM转移的模型描述了所观察到的效应。获得的结果表明,在存在弱酸和弱碱梯度的情况下,在质子载体存在时,包括生物膜在内的膜上可能会出现电位。

相似文献

1
[Generation of potential on bilayer lipid membrane in proton combination-separation reactions in the layers adjacent to the membranes].[质子在与膜相邻层中的结合-分离反应中双层脂质膜上电势的产生]
Biofizika. 1982 Jul-Aug;27(4):635-40.
2
Generation of potential in lipid bilayer membranes as a result of proton-transfer reactions in the unstirred layers.未搅拌层中质子转移反应导致脂质双分子层膜中电位的产生。
J Bioenerg Biomembr. 1982 Dec;14(5-6):457-65. doi: 10.1007/BF00743071.
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[A model of non-electrogenic cation transport through bilayer lipid membranes].[一种通过双层脂质膜的非电生性阳离子转运模型]
Biofizika. 1984 Mar-Apr;29(2):232-6.
4
[The effect of the composition of phospholipid bilayer membranes on the steady state kinetic transfer of protons].
Biokhimiia. 1993 Jul;58(7):987-96.
5
Permeation of ammonia across bilayer lipid membranes studied by ammonium ion selective microelectrodes.用铵离子选择性微电极研究氨在双层脂质膜中的渗透。
Biophys J. 1997 May;72(5):2187-95. doi: 10.1016/S0006-3495(97)78862-3.
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[The weak acids as proton carriers through bilayer lipid membranes].[作为质子载体穿过双层脂质膜的弱酸]
Fiziol Zh (1994). 2007;53(1):48-54.
7
[Measurements of non-electrogenic fluxes through lipid bilayers induced by Men+/nH+-exchangers].
Biofizika. 1983 Nov-Dec;28(6):1022-5.
8
A new method of the measurement of the electrically neutral fluxes of cations through lipid bilayer membranes induced by Men+/nH+-exchangers.一种测量由Men+/nH+交换体诱导的阳离子通过脂质双分子层膜的电中性通量的新方法。
FEBS Lett. 1983 Oct 31;163(1):42-5. doi: 10.1016/0014-5793(83)81158-2.
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Measurements of local pH changes near bilayer lipid membrane by means of a pH microelectrode and a protonophore-dependent membrane potential. Comparison of the methods.利用pH微电极和质子载体依赖性膜电位测量双层脂质膜附近的局部pH变化。方法比较。
Biochim Biophys Acta. 1991 Nov 18;1070(1):279-82. doi: 10.1016/0005-2736(91)90176-9.
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Weak acid transport across bilayer lipid membrane in the presence of buffers. Theoretical and experimental pH profiles in the unstirred layers.在缓冲液存在下弱酸跨双层脂质膜的转运。未搅拌层中的理论和实验pH曲线。
Biophys J. 1993 Jun;64(6):1701-10. doi: 10.1016/S0006-3495(93)81542-X.