[Generation of potential on bilayer lipid membrane in proton combination-separation reactions in the layers adjacent to the membranes].

Addition of uncoupler at the background of concentration gradient of the salts of weak acids and bases (sodium acetate and ammonium chloride) brought about formation of the positive potential on the BLM part with high sodium acetate concentration and of the negative potential on the BLM part with high ammonium chloride concentration. It was shown that the potential formation was conditioned by the initiation of pH gradient in unstirred layers. The effects observed are described by means of the earlier developed [7] model of the transfer of weak acids and bases through BLM. The results obtained show that with the existence of gradients of weak acids and bases potentials may appear on the membrane, biological ones included, in the presence of protonophor.