Elin R J, Johnson E, Chesler R
Clin Chem. 1982 Oct;28(10):2098-100.
Uric acid as measured in serum by three different uricase (EC 1.7.3.3) methods (aca, Ektachem, and SMAC) and by the SMAC method with phosphotungstic acid was compared with a candidate Reference Method for uric acid. Serum specimens from 83 patients (uric acid concentrations, 19 to 141 mg/L) were analyzed by all five methods. Results were compared by using linear regression analysis, and the mean difference between results by the candidate Reference Method and the four other methods was calculated. Compared with the candidate Reference Method, the aca method gave the smallest deviation from zero for the intercept and the smallest mean difference, and the SMAC phosphotungstic acid method showed a slope closest to unity. The SMAC uricase method had the largest intercept and greatest deviation of the slope from unity.
采用三种不同的尿酸酶（EC 1.7.3.3）方法（aca法、Ektachem法和SMAC法）以及含磷钨酸的SMAC法测定血清中的尿酸，并将其与尿酸候选参考方法进行比较。用这五种方法对83例患者的血清标本（尿酸浓度为19至141mg/L）进行了分析。通过线性回归分析比较结果，并计算候选参考方法与其他四种方法结果之间的平均差异。与候选参考方法相比，aca法的截距与零的偏差最小，平均差异也最小，而SMAC磷钨酸法的斜率最接近1。SMAC尿酸酶法的截距最大，斜率与1的偏差也最大。
