Duncan P H, Gochman N, Cooper T, Smith E, Bayse D
Clin Chem. 1982 Feb;28(2):284-90.
We describe our optimization and evaluation of a candidate Reference Method for uric acid in serum. Reaction parameters were optimized for a manual, enzymic method for uric acid in which highly purified microbial uricase is used to quantitate uric acid by a differential ultraviolet procedure. We evaluated the method in terms of freedom from interferences, analytical recovery, precision, and comparison with five other uric acid methods. We conclude that (a) the candidate uric acid Reference Method exhibits the least interference; (b) all six methods exhibit satisfactory analytical recoveries and precision; and (c) results by all six methods agree well. As a result of this evaluation study, the manual ultraviolet uricase method for uric acid, with Tris as buffer, was chosen as the candidate Reference Method for uric acid.
我们描述了血清尿酸候选参考方法的优化及评估。针对一种手动酶法对尿酸反应参数进行了优化，该酶法使用高度纯化的微生物尿酸酶，通过差分紫外法对尿酸进行定量。我们从无干扰性、分析回收率、精密度以及与其他五种尿酸方法的比较等方面对该方法进行了评估。我们得出以下结论：（a）候选尿酸参考方法干扰最少；（b）所有六种方法的分析回收率和精密度均令人满意；（c）所有六种方法的结果一致性良好。作为这项评估研究的结果，选择了以Tris作为缓冲液的手动紫外尿酸酶法作为尿酸候选参考方法。
