Leung F Y, Henderson A R
Clin Chem. 1982 Oct;28(10):2139-43.
This method for determining aluminum in serum and urine is essentially free from matrix interference and gives a linear response with concentration to at least 500 micrograms/l. Use of a stabilized temperature platform (L'vov platform, Perkin-Elmer Corp.) to approach a "steady-state" temperature, addition of matrix modifiers [especially Mg(NO3)2], and the use of peak area integration all helped substantially diminish spectral interference. With the platform furnace, serum protein concentrations as great as 260 g/L did not interfere with the determination of Al. The within- and between-assay precision (CV) was less than or equal to 3.5% and less than or equal to 7.4%, respectively. Analytical recovery of Al added to serum ranged between 95 and 101% throughout the linear calibration range (to 500 micrograms/L), either when measured directly from the standard curve or by the method of standard additions. The reference interval for Al in 28 healthy subjects was 2-14 micrograms/L (mean 6.5, SD 4.1 micrograms/L), and for 130 patients on hemodialysis, 20-550 micrograms/L (mean 87.5, SD 62.5 micrograms/L).
这种测定血清和尿液中铝的方法基本不受基质干扰，在浓度至少为500微克/升时呈线性响应。使用稳定温度平台（L'vov平台，珀金埃尔默公司）来达到“稳态”温度、添加基质改性剂[尤其是Mg(NO3)2]以及使用峰面积积分都极大地有助于减少光谱干扰。使用平台炉时，高达260克/升的血清蛋白浓度不会干扰铝的测定。批内和批间精密度（CV）分别小于或等于3.5%和小于或等于7.4%。在整个线性校准范围内（至500微克/升），无论是直接从标准曲线测量还是通过标准加入法测量，添加到血清中的铝的分析回收率在95%至101%之间。28名健康受试者的铝参考区间为2 - 14微克/升（均值6.5，标准差4.1微克/升），130名血液透析患者的参考区间为20 - 550微克/升（均值87.5，标准差62.5微克/升）。
