对胎儿和成年兔子心房及心室心肌肌球蛋白的电泳分析。

Electrophoretic analyses of atrial and ventricular cardiac myosins from foetal and adult rabbits.

Srihari T, Tuchschmid C R, Hirzel H O, Schaub M C

Comp Biochem Physiol B. 1982;72(3):353-7. doi: 10.1016/0305-0491(82)90212-7.

Abstract

Rabbit cardiac myosins from atrium and ventricle were found to differ in their native form in pyrophosphate gel electrophoresis as well as in their light chain pattern in one- and two-dimensional electrophoretic systems. 2. The myosin light chain pattern in sodium dodecylsulfate gel electrophoresis differs between atrial and ventricular tissues in the mammalians (rabbit, dog, human, pig, sheep and rat) but not in the chicken. 3. In foetal rabbit ventricle an additional light chain is observed which, in two-dimensional electrophoresis with adult cardiac light chains, was found to be the same as atrial light chain-1.

摘要

研究发现，兔心房和心室的心肌肌球蛋白在焦磷酸凝胶电泳中的天然形式以及在一维和二维电泳系统中的轻链模式存在差异。2. 在哺乳动物（兔、狗、人、猪、羊和大鼠）中，十二烷基硫酸钠凝胶电泳中的肌球蛋白轻链模式在心房和心室组织之间有所不同，但在鸡中则没有差异。3. 在胎儿兔心室中观察到一条额外的轻链，在与成年心脏轻链进行二维电泳时，发现它与心房轻链-1相同。