三种仓鼠物种的DNA/DNA比较重缔合动力学

Comparative DNA/DNA reassociation kinetics in three hamster species.

Leipoldt M, Eckhardt R, Schmid M

Comp Biochem Physiol B. 1982;72(3):385-91. doi: 10.1016/0305-0491(82)90216-4.

Abstract

Three major DNA repetition classes can be distinguished in the genomes of three hamster species Mesocricetus auratus, Cricetulus griseus, and Phodopus sungorus sungorus: A very fast reassociating fraction of about 13% of the DNA, a fast reassociating fraction comprising 10-23% of the DNA, and a slowly reassociating fraction containing single-copy sequences of 63-78% of hamster DNA. 2. In the DNA of the Syrian and Djungarian hamster 43%-53%, and in the DNA of the Chinese hamster 80% of the single-copy sequences are interspersed with repetitive sequences. 3. The lengths of repetitive DNA sequences, vary between more than 3 kb and 0.3 kb, with the majority of sequences having a length of 0.3 kb to 0.5 kb. 4. The findings suggest that the genomes of the hamster species studied are organized in a short period interspersion pattern. 5. Unlike other vertebrate and invertebrate genomes, moderately repetitive sequences exhibit a high degree of intraspecific homology in the hamster genome.

摘要

在三种仓鼠物种，即金黄仓鼠、中国仓鼠和黑线仓鼠的基因组中，可以区分出三大类DNA重复序列：约占DNA 13%的非常快速复性部分、占DNA 10 - 23%的快速复性部分以及包含仓鼠DNA 63 - 78%单拷贝序列的缓慢复性部分。2. 在叙利亚仓鼠和加卡利亚仓鼠的DNA中，43% - 53%的单拷贝序列与重复序列相间分布，而在中国仓鼠的DNA中，80%的单拷贝序列与重复序列相间分布。3. 重复DNA序列的长度在超过3 kb到0.3 kb之间变化，大多数序列的长度为0.3 kb至0.5 kb。4. 这些发现表明，所研究的仓鼠物种的基因组是以短周期散布模式组织的。5. 与其他脊椎动物和无脊椎动物的基因组不同，中度重复序列在仓鼠基因组中表现出高度的种内同源性。