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叙利亚仓鼠(金仓鼠)和黑线毛足鼠染色体上核仁组织区的定位

Location of nucleolar organizing regions on the chromosomes of the Syrian hamster (Mesocricetus auratus) and the Djungarian hamster (Phodopus sungorus).

作者信息

Bigger T R, Savage J R

出版信息

Cytogenet Cell Genet. 1976;16(6):495-504. doi: 10.1159/000130667.

Abstract

Terminal chromosome associations ("satellite associations") in ASG-banded preparations have been used to determine the number and location of staellites in the Syrian hamster (Mesocricetus auratus) and the Djungarian hamster (Phodopus sungorus). Five pairs of satellites are found in the former and four in the latter. Nucleolar organizing regions (NORs) were visualized with the Ag-AS silver precipitation technique, and their number and position corresponded exactly with the number and position of satellites in Phodopus, where positive chromosome identification can be made in the absence of banding. Numerical agreement is exact in Mesocricetus as well, and the morphology of the silver-tagged NOR-bearing chromosomes strongly suggests that corrrespondence also occurs in this species.

摘要

在ASG显带制片中,端粒染色体联会(“随体联会”)已被用于确定叙利亚仓鼠(金仓鼠)和黑线毛足鼠中随体的数量和位置。在前者中发现了五对随体,在后者中发现了四对。用Ag-AS银沉淀技术观察核仁组织区(NORs),其数量和位置与黑线毛足鼠中随体的数量和位置完全对应,在该物种中,即使没有显带也能准确鉴定染色体。在金仓鼠中数量也完全一致,并且带有银标记的含NOR染色体的形态强烈表明该物种中也存在对应关系。

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