Kelbaek H
Eur J Nucl Med. 1986;12(3):107-9. doi: 10.1007/BF00276700.
The role of the leukocyte isolation procedure on cell labelling with 99mTc has been evaluated. Separation of leukocytes was performed by two procedures: sedimentation on methyl cellulose, followed by discontinuous gradient centrifugation; methyl cellulose sedimentation and hypotonic haemolysis of residual red blood cells. After washing the cells in saline and incubation with a stannous pyrophosphate agent, the leukocytes were labelled with 5-10 mCi 99mTc. Procedure 1 gave a higher purity but lower recovery of polymorphonuclear leukocytes, and a minor contamination of red blood cells. 99mTc labelling of cells was slightly more efficient with this method, probably due to the presence of red blood cells. Procedure 1 is recommended for in vitro studies on cell kinetics and procedure 2 is recommended for clinical use.
已经评估了白细胞分离程序对用99mTc进行细胞标记的作用。白细胞的分离通过两种程序进行：在甲基纤维素上沉降，然后进行不连续梯度离心；甲基纤维素沉降以及残留红细胞的低渗溶血。在用盐水洗涤细胞并与焦磷酸亚锡试剂孵育后，白细胞用5 - 10毫居里的99mTc进行标记。程序1得到的多形核白细胞纯度较高但回收率较低，且红细胞污染较少。用这种方法对细胞进行99mTc标记效率略高，可能是由于存在红细胞。程序1推荐用于细胞动力学的体外研究，程序2推荐用于临床应用。
