Schwartz E R, Sugumaran G
In Vitro. 1982 Mar;18(3 Pt 1):254-60. doi: 10.1007/BF02618579.
Chondrocytes derived from normal human adult articular cartilage were established and maintained for over 5 months in a completely defined medium without the addition of serum or any other growth factors. At the end of 5 months, these cells were still metabolically active. The cells incorporated [3H] thymidine into DNA, incorporated [35S] sulfate into proteoglycans, and exhibited lysosomal enzyme activities. The 35S-labeled proteoglycans isolated from the culture medium had elution profiles on high pressure liquid chromatography (HPLC) similar to those observed for proteoglycans from other mammalian sources. This self-contained growth competence may reflect a need produced by the unusual avascular and alymphatic character of articular cartilage.
从正常成人人类关节软骨中分离出的软骨细胞，在完全限定培养基中培养并维持了5个多月，期间未添加血清或任何其他生长因子。5个月末，这些细胞仍具有代谢活性。细胞将[3H]胸腺嘧啶核苷掺入DNA，将[35S]硫酸盐掺入蛋白聚糖，并表现出溶酶体酶活性。从培养基中分离出的35S标记的蛋白聚糖在高压液相色谱（HPLC）上的洗脱图谱与从其他哺乳动物来源的蛋白聚糖所观察到的相似。这种自我维持的生长能力可能反映了关节软骨异常的无血管和无淋巴管特性所产生的需求。
