Deichman G I, Kluchareva T E, Kashkina L M, Matveeva V A, Vendrov E L, Zemtsova V I
Int J Cancer. 1982 Sep 15;30(3):349-53. doi: 10.1002/ijc.2910300315.
In our previous experiments in Syrian hamsters the in vivo semiquantitative method of determination of the natural resistance-depressing (NRD) activity of tumor cells was described. It was demonstrated that inactivated cells of different tissue culture cell lines derived from in vivo tumors in contrast to normal, or in vitro spontaneously transformed hamster embryo (STHE) cells, bear the NRD factor. It was suggested that NRD activity of tumor cells might be essential for the development of primary tumors and metastases (Deichman et al., 1979). Therefore, in this study we compared the NRD activity and metastatic activity (MA) of the parental STHE cells never passaged in vivo and STHE in vivo sublines, obtained from subcutaneous tumor nodules and from individual spontaneous lung metastases (LM) after s.c. transplantation of STHE cells. Five LM sublines of STHE strain (out of eight investigated) were NRD active as well as the cells of one of two s.c. tumor nodules. Study of MA, i.e. the relation between the number of cells inoculated retroorbitally in the bloodstream of hamsters and the number of experimental metastases developed in the lungs of inoculated animals, demonstrated that the highest MA was observed with the cells of LM sublines possessing the highest NRD activity. Parental STHE cells did not depress natural anti-tumor resistance and their MA was the lowest. The data presented demonstrate the heterogeneity of the population of constantly cultured in vitro STHE cells in relation to their NRD activity and the selection of NRD active cells during the development of lung metastases.
在我们之前对叙利亚仓鼠进行的实验中，描述了一种体内半定量测定肿瘤细胞自然抗性降低（NRD）活性的方法。结果表明，与正常的或体外自发转化的仓鼠胚胎（STHE）细胞相比，源自体内肿瘤的不同组织培养细胞系的灭活细胞携带NRD因子。有人提出肿瘤细胞的NRD活性可能对原发性肿瘤和转移瘤的发展至关重要（戴克曼等人，1979年）。因此，在本研究中，我们比较了从未在体内传代的亲代STHE细胞以及从皮下肿瘤结节和皮下移植STHE细胞后个体自发肺转移灶（LM）获得的STHE体内亚系的NRD活性和转移活性（MA）。STHE品系的五个LM亚系（在八个研究对象中）以及两个皮下肿瘤结节之一的细胞具有NRD活性。对MA的研究，即经眶后接种到仓鼠血流中的细胞数量与接种动物肺部形成的实验性转移瘤数量之间的关系表明，NRD活性最高的LM亚系细胞的MA最高。亲代STHE细胞不会降低天然抗肿瘤抗性，其MA最低。所呈现的数据表明，持续体外培养的STHE细胞群体在NRD活性方面存在异质性，并且在肺转移发展过程中会选择具有NRD活性的细胞。
