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苜蓿中华根瘤菌温和噬菌体16-3结构蛋白的鉴定

Identification of structural proteins of Rhizobium meliloti temperate phage 16-3.

作者信息

Erdei S, Dudás B, Orosz L, Duda E

出版信息

J Gen Virol. 1982 Sep;62 (Pt 1):145-52. doi: 10.1099/0022-1317-62-1-145.

Abstract

The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis. Five major and five minor proteins were identified and characterized with respect to their size, isoelectric point and their distribution between the head ad tail of the phage particle. The synthesis of structural proteins was studied by one- and two-dimensional gel electrophoresis.

摘要

利用聚丙烯酰胺凝胶电泳、等电聚焦和琼脂糖凝胶电泳对苜蓿根瘤菌温和噬菌体16 - 3的结构蛋白进行了分析。鉴定出了5种主要蛋白和5种次要蛋白,并对其大小、等电点以及在噬菌体颗粒头部和尾部的分布进行了表征。通过一维和二维凝胶电泳研究了结构蛋白的合成。

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