Esche H, Schweiger M, Trautner T A
Mol Gen Genet. 1975 Dec 23;142(1):45-55.
A total of 23 phage specific proteins (including four head and six tail proteins) could be identified after SDS polyacrylamide gel electrophoresis of extracts from phage SPP1 infected Bacillus subtilis cells. The total molecular weight of the proteins amounts to approximately 1.9 X 10(6) daltons, equivalent to the majority of the coding capacity of SPP1 DNA. It can thus be assumed that almost all SPP1 coded proteins have been identified. Protein assignments to phage cistrons were made by analysis of extracts from nonpermissive cells infected with sus-mutants. The SPP1 specified proteins can be subdivided into three groups on the basis of the time of their synthesis during the latent period. Host protein synthesis is not significantly affected by SPP1 infection. Normal expression of host genes appears to be essential for SPP1 growth.
对噬菌体SPP1感染的枯草芽孢杆菌细胞提取物进行SDS聚丙烯酰胺凝胶电泳后，总共可鉴定出23种噬菌体特异性蛋白（包括4种头部蛋白和6种尾部蛋白）。这些蛋白的总分子量约为1.9×10⁶道尔顿，相当于SPP1 DNA的大部分编码能力。因此可以假定，几乎所有由SPP1编码的蛋白都已被鉴定出来。通过分析感染了sus突变体的非允许细胞的提取物，将蛋白分配到噬菌体顺反子上。根据潜伏期内合成时间的不同，SPP1指定的蛋白可分为三组。宿主蛋白的合成不受SPP1感染的显著影响。宿主基因的正常表达似乎对SPP1的生长至关重要。
