Chanda P K, Chatterjee S N
Acta Virol. 1976 Aug;20(4):261-6.
Cholera phage PL 163/10, belonging to Mukerjee's Group I, was purified by alternate cycles of low and high speed centrifugation. Gel electrophoresis revealed the presence of four polypeptide chains of respective molecular weights of 10,370+/-515 (A), 30,000+/-1,303 (B), 40,000+/-1,049(C) and 64,000+/-2,433 (D) daltons. Electrophoresis of the sample alkylated with iodoacetic acid resolved the presence of only one polypeptide chain of an average molecular weight of 10,310+/-565 daltons. The polypeptides B, C and D could be interpreted as trimer, tetramer and hexamer respectively of the polypeptide A, which was the basic structural protein of this phage.
属于穆克吉第一组的霍乱噬菌体PL 163/10通过低速和高速离心交替循环进行纯化。凝胶电泳显示存在四条多肽链，其分子量分别为10,370±515（A）、30,000±1,303（B）、40,000±1,049（C）和64,000±2,433（D）道尔顿。用碘乙酸烷基化的样品进行电泳，结果显示仅存在一条平均分子量为10,310±565道尔顿的多肽链。多肽B、C和D可分别解释为多肽A的三聚体、四聚体和六聚体，多肽A是该噬菌体的基本结构蛋白。
