Tas J, Berndsen R G
J Histochem Cytochem. 1977 Sep;25(9):1058-62. doi: 10.1177/25.9.71326.
Microspectrophotometric analyses combined with model experiments using polyacrylamide films containing different types of purified glycosaminoglycan or glycosaminoglycan and basic protein, have been applied to investigate the chemical characteristics of the glycosaminoglycan moiety present in rat mucosal mast cell granules. The results obtained with these histochemical techniques present evidence of the absence of significant amounts of heparin and the presence of lower sulfated glycosaminoglycan(s) in the granules of these cells.
微分光光度分析结合使用含有不同类型纯化糖胺聚糖或糖胺聚糖与碱性蛋白的聚丙烯酰胺膜进行的模型实验,已被用于研究大鼠黏膜肥大细胞颗粒中存在的糖胺聚糖部分的化学特性。通过这些组织化学技术获得的结果表明,这些细胞的颗粒中不存在大量肝素,而是存在较低硫酸化程度的糖胺聚糖。