Harder D, Virsik P
Strahlentherapie. 1982 Jul;158(7):440-3.
The well-known fact that the dose dependence of cell survival, when measured by the macrocolony-forming test, can be fitted with the multitarget expression is re-investigated by considering the delayed loss of cellular reproductivity in a microcolony. As the reproductive capacity of each single cell of the microcolony must be lost in order to prevent the development of a macrocolony, the reproductive death of a microcolony is in mathematical analogy to the multitarget model and provides the biological explanation for its formal applicability. Published data from colony-size distributions and the model of "latent genetical damage" are utilized to understand the exponential dose dependence of single cell survival in a microcolony. Exponential survival curves obtained at high LET are explained by an undelayed loss of reproductivity in the irradiated cell. The restitution of the "shoulder" in delayed-plating and split-dose experiments is interpreted by repair effects.
通过考虑微克隆中细胞繁殖能力的延迟丧失，对一个众所周知的事实进行了重新研究，即当通过大克隆形成试验测量时，细胞存活的剂量依赖性可以用多靶标表达式来拟合。由于必须丧失微克隆中每个单细胞的繁殖能力才能阻止大克隆的形成，微克隆的繁殖死亡在数学上类似于多靶标模型，并为其形式上的适用性提供了生物学解释。利用来自克隆大小分布的已发表数据和“潜在遗传损伤”模型来理解微克隆中单个细胞存活的指数剂量依赖性。高传能线密度（LET）下获得的指数存活曲线可以通过受辐照细胞中繁殖能力的无延迟丧失来解释。延迟接种和分次剂量实验中“肩区”的恢复可以用修复效应来解释。
