Komel R
Z Allg Mikrobiol. 1982;22(6):373-8. doi: 10.1002/jobm.3630220604.
The rate of transformation of cholesterol by Nocardia sp. is affected by the culture medium composition as well as by the way it has been treated before inoculation. When 1% of peptone was sterilized separately and afterwards added to the basic medium, the transformation of cholesterol to bisnorchola-1,4-dienic acid, as well as further aerobic degradation, was accelerated by a factor of 2. If this enriched medium was kept at rest overnight, the rate of transformation was lowered to half. Heating of additional peptone together with a basic medium resulted only in a slight diminution of the transformation of the preceding 4-ene metabolite to the final 1,4-diene when compared with a normal basic medium without any additive.
诺卡氏菌属对胆固醇的转化速率受培养基成分以及接种前处理方式的影响。当1%的蛋白胨单独灭菌后再添加到基础培养基中时，胆固醇向双降胆甾-1,4-二烯酸的转化以及进一步的需氧降解加速了2倍。如果将这种富集培养基静置过夜，转化速率会降至一半。与没有任何添加剂的正常基础培养基相比，将额外的蛋白胨与基础培养基一起加热只会使前一个4-烯代谢产物向最终的1,4-二烯的转化略有减少。
