Lober M, Krantz S, Herrmann I
Acta Biol Med Ger. 1982;41(5):487-96.
Methods for the production of antibodies against Paraoxon in rabbits are described. The highest-titre antisera were produced with conjugates containing a succinyl spacer group with a degree of derivatisation between 8 and 12 haptens per albumin molecule. The antibody response was tested by immunoprecipitation in agar gel with a hapten-rabbit-albumin-complex and by a radioimmunoassay. The synthesis of 125I labelled tracers is reported. The most potent antisera bound 50% of the radioactive tracer after 1:10(5) dilution and showed apparent affinity constants of 10(6) to 10(7) M-1 for Paraoxon. Compared to Paraoxon these antisera possessed cross reactivities with Parathion of 8.8 to 13.8%, with Methylparathion of 0.35 to 0.82%, and with Dimethoat of 0.0006 to 0.0012%.
描述了在兔中产生抗对氧磷抗体的方法。使用每分子白蛋白具有8至12个半抗原的琥珀酰间隔基团的缀合物产生了最高滴度的抗血清。通过用半抗原-兔-白蛋白复合物在琼脂凝胶中进行免疫沉淀和放射免疫测定来测试抗体反应。报道了125I标记示踪剂的合成。最有效的抗血清在1:10(5)稀释后结合了50%的放射性示踪剂，并且对氧磷的表观亲和常数为10(6)至10(7) M-1。与对氧磷相比，这些抗血清与对硫磷的交叉反应性为8.8至13.8%，与甲基对硫磷的交叉反应性为0.35至0.82%，与乐果的交叉反应性为0.0006至0.0012%。
