Reisser D, Boucrot P
Lipids. 1978 Nov;13(11):796-800. doi: 10.1007/BF02533478.
The addition of total phospholipids, phosphatidylcholines, triglycerides, cholesterol or glycerol to incubation media containing rat pancreatic juice and bile labeled with [9, 10 3H2] oleic acid (90% of the radioactivity present as phospholipids) had no effect on the hydrolysis of bile endogenous phospholipids. The introduction of 2 or 10 mg of phosphatidylcholines and 0.5 ml of bile (approximately 1.5 mg of phospholipids) into the rat upper duodenum decreased the rate absorption of native bile phospholipids. It was not followed by an increase of free fatty acids released from biliary phospholipids in the intestinal lumen. The introduction of bile (0.5 ml) and small amounts of triolein (1.4--3.5 mg) into the duodenum had little effect on the rate of hydrolysis and absorption of native bile phospholipids, but caused a reduced absorption of the free fatty acids released or those coming from initial nonphosphorus biliary lipids. The introduction of bile (0.5 ml) and large amounts of triolein (30 mg) into the duodenum increased the rates of hydrolysis and absorption of endogenous bile phospholipids. These observations suggest that luminal lipid components can modify the organization of luminal micelles and, consequently, the action of the pancreatic phospholipase A2 and the absorption of bile lipids.
向含有用[9, 10 3H2]油酸（90%的放射性以磷脂形式存在）标记的大鼠胰液和胆汁的孵育培养基中添加总磷脂、磷脂酰胆碱、甘油三酯、胆固醇或甘油，对胆汁内源性磷脂的水解没有影响。将2或10毫克磷脂酰胆碱和0.5毫升胆汁（约1.5毫克磷脂）引入大鼠十二指肠上段，会降低天然胆汁磷脂的吸收速率。随后肠腔内胆汁磷脂释放的游离脂肪酸并未增加。将胆汁（0.5毫升）和少量三油酸甘油酯（1.4 - 3.5毫克）引入十二指肠，对天然胆汁磷脂的水解和吸收速率影响不大，但会导致释放的游离脂肪酸或来自初始非磷胆汁脂质的游离脂肪酸的吸收减少。将胆汁（0.5毫升）和大量三油酸甘油酯（30毫克）引入十二指肠，会增加内源性胆汁磷脂的水解和吸收速率。这些观察结果表明，肠腔内脂质成分可以改变肠腔内微胶粒的结构，从而改变胰磷脂酶A2的作用和胆汁脂质的吸收。
