短链脂肪酸对丝裂原刺激的和白血病淋巴细胞中脱氧胸苷掺入DNA的抑制作用。

Inhibitory effect of short-chain aliphatic acids on deoxythymidine incorporation into DNA in mitogen-stimulated and leukemic lymphocytes.

作者信息

Stolc V

出版信息

Am J Hematol. 1982 Sep;13(2):111-20. doi: 10.1002/ajh.2830130203.

Abstract

The effect of short-chain aliphatic acids on [3H]deoxythymidine incorporation into DNA was studied in human mitogen-stimulated lymphocytes. Butyric acid at 1-2 mM level was strongly inhibitory; however, its hydroxy or amino derivatives were ineffective. Valeric and propionic acids were less inhibitory. Formic, acetic, and hexanoic acids did not have any inhibitory effect. The effect of butyrate persisted in washed lymphocytes for 24-48 hr depending on the concentration used and the time of its addition into the incubation medium. The inhibitory effects of butyrate, valerate, and propionate on deoxythymidine and amino acid incorporation into DNA and protein were also found in human leukocytes in myelocytic and lymphocytic leukemias.

摘要

在人有丝分裂原刺激的淋巴细胞中研究了短链脂肪酸对[3H]脱氧胸苷掺入DNA的影响。1-2 mM浓度的丁酸具有强烈的抑制作用；然而，其羟基或氨基衍生物则无效。戊酸和丙酸的抑制作用较小。甲酸、乙酸和己酸没有任何抑制作用。丁酸盐的作用在洗涤过的淋巴细胞中持续24-48小时，这取决于所用的浓度及其添加到培养液中的时间。在髓细胞性白血病和淋巴细胞性白血病患者的白细胞中也发现了丁酸盐、戊酸盐和丙酸盐对脱氧胸苷和氨基酸掺入DNA及蛋白质的抑制作用。