Handelsman B, Bonorris G, Marks J W, Schoenfield L J
Am J Physiol. 1982 Nov;243(5):G424-7. doi: 10.1152/ajpgi.1982.243.5.G424.
Three groups of golden Syrian hamsters were fed equimolar amounts of taurine-conjugated ursodeoxycholic acid (TUDCA) or unconjugated ursodeoxycholic acid (UDCA) with or without excess taurine for 2 wk. They also received a lithogenic diet composed of standard rodent chow containing ethynylestradiol and increased cholesterol. Bile was obtained from the gallbladder after ketamine anesthesia and analyzed for biliary lipids. The percentage of biliary UDCA was higher with TUDCA (38.5 +/- 3.7) than with UDCA plus taurine (26.5 +/- 2.0, P less than 0.01). The glycine-to-taurine ratio of biliary UDCA conjugates was lower with TUDCA (0.9 +/- 0.1) than with UDCA plus taurine (2.1 +/- 0.2, P less than 0.01) and was highest with UDCA without taurine (4.1 +/- 0.1, P less than 0.01). Biliary cholesterol (molar percentage) and the cholesterol saturation indices with or without correction for UDCA-rich bile were significantly lower with TUDCA than with unconjugated UDCA with or without added taurine. In conclusion, administration for 2 wk of TUDCA, compared with an equimolar amount of unconjugated UDCA plus taurine, produced in the bile of hamsters a higher percentage of UDCA, a lower glycine-to-taurine ratio of UDCA conjugates, and a lower saturation index before and after adjustment for UDCA-rich bile.
将三组金黄叙利亚仓鼠分别用等摩尔量的牛磺酸结合型熊去氧胆酸（TUDCA）或非结合型熊去氧胆酸（UDCA）喂养2周，其中两组分别添加或不添加过量牛磺酸。它们还接受了由含乙炔雌二醇和增加胆固醇的标准啮齿动物饲料组成的致石性饮食。在氯胺酮麻醉后从胆囊获取胆汁，并分析胆汁脂质。TUDCA组胆汁中UDCA的百分比（38.5±3.7）高于UDCA加牛磺酸组（26.5±2.0，P<0.01）。TUDCA组胆汁中UDCA结合物的甘氨酸与牛磺酸比值（0.9±0.1）低于UDCA加牛磺酸组（2.1±0.2，P<0.01），且在不添加牛磺酸的UDCA组中最高（4.1±0.1，P<0.01）。TUDCA组胆汁胆固醇（摩尔百分比）以及校正富含UDCA胆汁前后的胆固醇饱和指数均显著低于添加或不添加牛磺酸的非结合型UDCA组。总之，与等摩尔量的非结合型UDCA加牛磺酸相比，给仓鼠投喂2周TUDCA后，其胆汁中UDCA百分比更高，UDCA结合物的甘氨酸与牛磺酸比值更低，且校正富含UDCA胆汁前后的饱和指数也更低。
