Sinclair J F, Smith L, Bement W J, Sinclair P R, Bonkowsky H L
Biochem Pharmacol. 1982 Sep 1;31(17):2811-5. doi: 10.1016/0006-2952(82)90138-1.
The amount of cytochrome P-450 was increased to different extents after treatment of cultured chick embryo hepatocytes with n-propanol, isopropanol, n-butanol, or isobutanol. These increases were associated with increases in benzphetamine demethylase activity, a cytochrome P-450-catalyzed oxidation, and glucuronidation of phenol red, catalyzed by UDP-glucuronyl transferase. The responses were similar to those obtained with ethanol or propylisopropylacetamide, which the phenobarbital-like inducers. Pretreatment of cells with cycloheximide prevented the increases in both cytochrome P-450 and glucuronidation of phenol red, indicating that protein synthesis was required for these responses.
用正丙醇、异丙醇、正丁醇或异丁醇处理培养的鸡胚肝细胞后，细胞色素P - 450的含量有不同程度的增加。这些增加与苄非他明脱甲基酶活性的增加以及UDP - 葡糖醛酸基转移酶催化的酚红葡糖醛酸化有关，苄非他明脱甲基酶活性是一种细胞色素P - 450催化的氧化反应。这些反应与用乙醇或丙基异丙基乙酰胺（苯巴比妥样诱导剂）所获得的反应相似。用环己酰亚胺对细胞进行预处理可阻止细胞色素P - 450的增加以及酚红的葡糖醛酸化，这表明这些反应需要蛋白质合成。
