White E, Welch V M, Sun T, Sniegoski L T, Schaffer R, Hertz H S, Cohen A
Biomed Mass Spectrom. 1982 Sep;9(9):395-405. doi: 10.1002/bms.1200090907.
Two isotope dilution mass spectrometric methods have been developed for the determination of D-glucose in human serum. Each uses a uniformly labeled (13C)glucose as the internal standard. The first method involves conversion of glucose into 1,2:5,6-di-O-isopropylidene-alpha-D-glucofuranose and an extensive clean-up, followed by quantitation using packed column gas chromatography mass spectrometry. In the second method, glucose is converted into alpha-D-glucofuranose cyclic 1,2:3,5-bis(butylboronate)-6-acetate. The wet chemistry work-up is simpler, but analysis by capillary gas chromatography mass spectrometry is required. Both methods exhibit excellent precision (coefficients of variation less than 0.3%) and provided mean values that agree within 1% for all serum pools tested.
已开发出两种同位素稀释质谱法用于测定人血清中的D-葡萄糖。每种方法都使用均匀标记的（13C）葡萄糖作为内标。第一种方法是将葡萄糖转化为1,2:5,6-二-O-异丙叉基-α-D-葡萄糖呋喃糖并进行大量净化，然后使用填充柱气相色谱质谱法进行定量。第二种方法是将葡萄糖转化为α-D-葡萄糖呋喃糖环状1,2:3,5-双（硼酸丁酯）-6-乙酸酯。湿化学处理更简单，但需要通过毛细管气相色谱质谱法进行分析。两种方法都具有出色的精密度（变异系数小于0.3%），并且对于所有测试的血清池，所提供的平均值在1%以内相符。
