Bell C R, Holder-Franklin M A, Franklin M
Can J Microbiol. 1982 Aug;28(8):959-75. doi: 10.1139/m82-144.
Forty-eight continuous culture enrichments were performed on summer and winter water samples from two contrasting rivers. The cell output from each chemostat was dependent on the temperature and nitrogen concentration of each enrichment. The diversity of the populations from the continuous cultures, as assessed by species diversity analysis, was always greater than populations obtained on agar plates. However, the species isolated exclusively by continuous culture in these experiments were not unique to the chemostat. All of these species had been isolated at some time on plates directly. High nitrogen concentrations were found to decrease diversity. Populations sampled during the winter were influenced primarily by the concentration of nitrogen. Summer isolates were affected mainly by the temperature of the culture medium. The nutritional versatility of the population was not affected by enrichment of the continuous culture.
对来自两条截然不同河流的夏季和冬季水样进行了48次连续培养富集实验。每个恒化器的细胞输出取决于每次富集的温度和氮浓度。通过物种多样性分析评估，连续培养物中菌群的多样性始终高于琼脂平板上获得的菌群。然而，在这些实验中仅通过连续培养分离出的物种并非恒化器所特有。所有这些物种都曾在某个时间直接从平板上分离出来。发现高氮浓度会降低多样性。冬季采样的菌群主要受氮浓度影响。夏季分离株主要受培养基温度影响。连续培养的富集并未影响菌群的营养多样性。
