Melnick R L, Schiller C M
Environ Health Perspect. 1982 Nov;45:51-6. doi: 10.1289/ehp.824551.
The effects of mono- and dibutyl phthalate and mono- and di(2-ethylhexyl) phthalate on energy-dependent K+ uptake, respiration rates, and succinate cytochrome c reductase activities of isolated rat liver mitochondria were evaluated. The energy-coupling processes, active K+ transport and oxidative phosphorylation, were affected most by di-n-butyl phthalate and mono(2-ethylhexyl) phthalate. Mono-n-butyl phthalate had a moderate effect on energy coupling and di(2-ethylhexyl) phthalate had no apparent effect. The potency of inhibition of succinate cytochrome c reductase activity was mono(2-ethylhexyl) phthalate greater than di-n-butyl phthalate greater than mono-n-butyl phthalate = di(2-ethylhexyl) phthalate. It is concluded that phthalate esters affect mitochondrial activities by altering the permeability properties of the inner membrane and by inhibiting succinate dehydrogenase activity.
评估了邻苯二甲酸单丁酯和二丁酯以及邻苯二甲酸单(2-乙基己基)酯和二(2-乙基己基)酯对分离的大鼠肝线粒体能量依赖性钾摄取、呼吸速率和琥珀酸细胞色素c还原酶活性的影响。能量偶联过程、活性钾转运和氧化磷酸化受邻苯二甲酸二正丁酯和邻苯二甲酸单(2-乙基己基)酯影响最大。邻苯二甲酸单正丁酯对能量偶联有中等影响,邻苯二甲酸二(2-乙基己基)酯无明显影响。抑制琥珀酸细胞色素c还原酶活性的效力为邻苯二甲酸单(2-乙基己基)酯>邻苯二甲酸二正丁酯>邻苯二甲酸单正丁酯 = 邻苯二甲酸二(2-乙基己基)酯。得出的结论是,邻苯二甲酸酯通过改变内膜的通透性和抑制琥珀酸脱氢酶活性来影响线粒体活性。