Elder G H, Wyvill P C
Enzyme. 1982;28(2-3):186-95. doi: 10.1159/000459101.
A sensitive, specific method for the measurement of uroporphyrinogen decarboxylase(porphyrinogen carboxy-lyase, EC4.1.1.37) in liver homogenates, haemolysates and other tissues is described. Pentacarboxylic porphyrinogen III, prepared from the corresponding porphyrin by reduction with sodium-mercury amalgam, is used as substrate. After oxidation and methyl esterification the reaction product, coproporphyrinogen III, is separated from the substrate and measured by high-performance liquid chromatography, with mesoporphyrin IX as internal standard. The method can readily be adapted for use with any of the other substrates of uroporphyrinogen decarboxylase, including uroporphyrinogens I and III.
本文描述了一种灵敏、特异的方法，用于测定肝匀浆、溶血产物及其他组织中的尿卟啉原脱羧酶（卟啉原羧基裂解酶，EC4.1.1.37）。由相应的卟啉经钠汞齐还原制备的五羧基卟啉原III用作底物。氧化和甲酯化后，将反应产物粪卟啉原III与底物分离，并以中卟啉IX为内标，通过高效液相色谱法进行测定。该方法可轻松适用于尿卟啉原脱羧酶的任何其他底物，包括尿卟啉原I和III。
