Gregg J H, Krefft M, Haas-Kraus A, Williams K L
Exp Cell Res. 1982 Nov;142(1):229-33. doi: 10.1016/0014-4827(82)90427-x.
A method was developed to obtain frozen sections of paraformaldehyde-fixed Dictyostelium slugs. These sections were ideal for studies using monoclonal antibodies. Two different monoclonal antibodies were used to recognize specific antigens on the surface of D. discoideum prespore cells by fluorescence microscopy. Neither of the two monoclonal antibodies bound to D. discoideum prestalk cells. D. mucoroides, a closely related species to D. discoideum, failed to bind either monoclonal antibody to its prespore cells. These results contrast with those obtained with either D. mucoroides [1] or D. discoideum [2] polyspecific antisera.
开发了一种用于获取经多聚甲醛固定的盘基网柄菌蛞蝓冷冻切片的方法。这些切片非常适合使用单克隆抗体进行研究。通过荧光显微镜，使用两种不同的单克隆抗体来识别盘基网柄菌前孢子细胞表面的特定抗原。这两种单克隆抗体均未与盘基网柄菌柄细胞结合。与盘基网柄菌亲缘关系密切的类毛霉网柄菌，其前孢子细胞也未与任何一种单克隆抗体结合。这些结果与使用类毛霉网柄菌[1]或盘基网柄菌[2]多特异性抗血清所获得的结果形成对比。
