噬菌体G4 DNA的核苷酸序列。

Nucleotide sequence of bacteriophage G4 DNA.

作者信息

Godson G N, Barrell B G, Staden R, Fiddes J C

出版信息

Nature. 1978 Nov 16;276(5685):236-47. doi: 10.1038/276236a0.

Abstract

The 5,577 nucleotide long sequence of bacteriophage G4 DNA has been determined using the 'plus and minus' and chain termination methods of DNA sequencing. This sequence has been compared with that of the closely related bacteriophage phiX174 (refs 1, 55). In the coding regions there is an average of 33.1% nucleotide sequence differences between the two genomes, but the distribution of these changes is not random and the sequence of some genes is more conserved than others. There is less sequence similarity between the untranslated intergenic regions of G4 and phiX174, but despite this the sequences of the J/F, F/G and H/A untranslated spaces in both genomes have similar sized hairpin loops, which may be related to their function.

摘要

利用DNA测序的“加减法”和链终止法，已确定了噬菌体G4 DNA的5577个核苷酸长的序列。该序列已与密切相关的噬菌体phiX174的序列进行了比较（参考文献1、55）。在编码区域，两个基因组之间平均有33.1%的核苷酸序列差异，但这些变化的分布并非随机，一些基因的序列比其他基因更保守。G4和phiX174的非翻译基因间区域之间的序列相似性较低，但尽管如此，两个基因组中J/F、F/G和H/A非翻译间隔区的序列具有大小相似的发夹环，这可能与其功能有关。