Reubi J C, Emson P C, Jessell T M, Iversen L L
Naunyn Schmiedebergs Arch Pharmacol. 1978 Oct;304(3):271-5. doi: 10.1007/BF00507968.
The effects of GABA, substance P and dopamine on the release of newly synthesized 3H-5-HT were investigated, using slices of rat substantia nigra superfused with L-3H-tryptophan in vitro. GABA (50 micron) had no inhibitory effect on the potassium-evoked-release of 3H-5-HT. Substance P (50 micron) and eledoisin (50 micron) stimulated the spontaneous release of 3H-5-HT. This effect seems to be indirect and is possibly mediated by dopaminergic neurones, since the dopamine antagonist drug alpha-flupenthixol (1 micron) abolished the substance P-evoked release of 5-HT. Furthermore, it was found that substance P (10 micron) stimulated 3H-dopamine release from nigral slices in vitro and the dopaminergic agonist apomorphine (50 micron) also stimulated 3H-5-HT release. Substance P may, therefore, activate nigral dopaminergic neurones which then release dopamine from their dendrites. The release of dopamine may in turn stimulate 5-HT release from terminals of the raphe-nigral pathway.
采用体外将L-3H-色氨酸灌注于大鼠黑质切片的方法，研究了γ-氨基丁酸（GABA）、P物质和多巴胺对新合成的3H-5-羟色胺（3H-5-HT）释放的影响。GABA（50微摩尔）对钾离子诱发的3H-5-HT释放无抑制作用。P物质（50微摩尔）和eledoisin（50微摩尔）刺激3H-5-HT的自发释放。这种作用似乎是间接的，可能由多巴胺能神经元介导，因为多巴胺拮抗剂阿立哌唑（1微摩尔）可消除P物质诱发的5-HT释放。此外，还发现P物质（10微摩尔）在体外刺激黑质切片释放3H-多巴胺，多巴胺能激动剂阿扑吗啡（50微摩尔）也刺激3H-5-HT释放。因此，P物质可能激活黑质多巴胺能神经元，然后这些神经元从其树突释放多巴胺。多巴胺的释放反过来可能刺激中缝黑质通路终末释放5-HT。
