多维液相色谱法：生理维生素K水平评估的一项突破。

Multidimensional liquid chromatography: a breakthrough in the assessment of physiological vitamin K levels.

作者信息

Lefevere M F, De Leenheer A P, Claeys A E, Claeys I V, Steyaert H

出版信息

J Lipid Res. 1982 Sep;23(7):1068-72.

Abstract

Owing to the lack of sensitivity and/or selectivity of the existing chemical assays, vitamin K deficiency has always been diagnosed indirectly by measuring its effect on blood coagulation. We used our recently developed multidimensional liquid chromatographic assay for what is, to the best of our knowledge, the first systematic investigation of physiological vitamin K levels in human blood. It allowed the unequivocal demonstration of trans-phylloquinone (vitamin K1) and its quantification down to a level of 0.5 nanogram per milliliter of serum (ng/ml. In healthy adults, a mean serum concentration of 2.6 ng/ml was found, with a normal range of 0.9 to 7.8 ng/ml. These values apparently are distributed in a log-normal way.

摘要

由于现有化学检测方法缺乏敏感性和/或选择性，维生素K缺乏症一直是通过测量其对血液凝固的影响来间接诊断的。据我们所知，我们使用最近开发的多维液相色谱检测方法，首次对人体血液中的生理维生素K水平进行了系统研究。该方法能够明确地检测出反式叶绿醌（维生素K1），并将其定量至血清中每毫升0.5纳克（ng/ml）的水平。在健康成年人中，血清平均浓度为2.6 ng/ml，正常范围为0.9至7.8 ng/ml。这些值显然呈对数正态分布。