Hockeborn M, Rick W
J Clin Chem Clin Biochem. 1982 Dec;20(12):921-7.
In the enzymic hydrolysis of triolein by lipase in serum, hydrogen ions are produced which lead to a decrease in the pH of the test system. The extent of this pH change depends on the catalytic activity of the lipase and on the buffering capacity of the test system. In the method described for the determination of the catalytic activity of lipase in serum, the decrease in pH is measured and recorded potentiometrically. Comparative studies showed a satisfactory correlation of results with those from the continuous titrimetric method. Further studies were performed with a modification of the detection method described by Härtel et al. ((1971) Z. Klin. Chem. Klin. Biochem. 9, 396-397), based on the estimation of the pH decrease from the colour change of an indicator. With operator experience, this test also gives reproducible results for the catalytic activity of lipase in serum.
在血清中脂肪酶对三油酸甘油酯的酶促水解过程中，会产生氢离子，这会导致测试系统的pH值下降。这种pH值变化的程度取决于脂肪酶的催化活性以及测试系统的缓冲能力。在所描述的测定血清中脂肪酶催化活性的方法中，pH值的下降是通过电位法进行测量和记录的。对比研究表明，结果与连续滴定法的结果具有良好的相关性。基于通过指示剂颜色变化估计pH值下降的方法，对Härtel等人（（1971年）《临床化学与临床生物化学杂志》9卷，396 - 397页）所描述的检测方法进行了改进，并开展了进一步研究。凭借操作人员的经验，该测试对于血清中脂肪酶的催化活性也能给出可重复的结果。
