Lütgens M, Gottschalk G
J Gen Microbiol. 1982 Aug;128(8):1915-21. doi: 10.1099/00221287-128-8-1915.
Out of 19 strains belonging to the family Enterobacteriaceae only Escherichia coli and Citrobacter strains fermented fumarate exclusively to succinate. This fermentation was dependent on the presence of molecular hydrogen or formate. The inability of these micro-organisms to convert fumarate to succinate, acetate and CO2 correlated with their lack, or low activity, of oxaloacetate decarboxylase. Continuous culture experiments were performed with Citrobacter freundii in minimal or complex medium with fumarate + H2 of formate, and the growth parameters were determined. From the data obtained, a Ymax fumarate dissimilated value of 10.5 +/- 0.8 g dry wt per mol fumarate dissimilated was calculated. This value demonstrates that, per mol fumarate reduced, at least 0.6 +/- 0.05 mol ATP is produced and subsequently used for biosynthetic purposes.
在属于肠杆菌科的19个菌株中，只有大肠杆菌和柠檬酸杆菌菌株能将富马酸盐完全发酵为琥珀酸盐。这种发酵依赖于分子氢或甲酸盐的存在。这些微生物无法将富马酸盐转化为琥珀酸盐、乙酸盐和二氧化碳，这与它们缺乏草酰乙酸脱羧酶或其活性较低有关。在含有富马酸盐+H2或甲酸盐的基本培养基或复合培养基中，用弗氏柠檬酸杆菌进行连续培养实验，并测定生长参数。根据获得的数据，计算出每摩尔被异化的富马酸盐的Ymax异化值为10.5±0.8克干重。该值表明，每摩尔被还原的富马酸盐至少产生0.6±0.05摩尔ATP，随后用于生物合成目的。
