幽门螺杆菌中的富马酸酯分解代谢

Fumarate catabolism in Helicobacter pylori.

作者信息

Mendz G L, Hazell S L

机构信息

School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia.

出版信息

Biochem Mol Biol Int. 1993 Oct;31(2):325-32.

PMID:8275020

Abstract

The metabolism of fumarate by Helicobacter pylori was investigated employing one- and two-dimensional 1H and 13C nuclear magnetic resonance spectroscopy. Metabolically competent cells generated malate and succinate from fumarate as the sole substrate indicating the presence of fumarase and fumarate reductase activities in the bacterium. In incubations of fumarate with cell lysates accumulation of lactate, acetate, formate and alanine was observed after the initial production of malate and succinate. The results indicate the existence of active fumarate catabolism in H. pylori and suggest the possibility of an ATP generating mechanism which may play an important role in the bioenergetics of the bacterium.

摘要

利用一维和二维氢谱和碳谱核磁共振波谱技术研究了幽门螺杆菌对富马酸盐的代谢情况。代谢活性良好的细胞以富马酸盐作为唯一底物生成苹果酸盐和琥珀酸盐，这表明该细菌中存在富马酸酶和富马酸还原酶活性。在用细胞裂解物孵育富马酸盐的过程中，在最初生成苹果酸盐和琥珀酸盐之后，观察到了乳酸盐、乙酸盐、甲酸盐和丙氨酸的积累。结果表明幽门螺杆菌中存在活跃的富马酸盐分解代谢，并提示可能存在一种ATP生成机制，该机制可能在该细菌的生物能量学中发挥重要作用。