Higgins J A
J Microsc. 1982 Oct;128(Pt 1):57-67. doi: 10.1111/j.1365-2818.1982.tb00437.x.
The endoplasmic reticulum has the enzymic machinery for the synthesis of both protein and phospholipid and hence plays a central role in its own biogenesis and that of other cellular membranes. The evidence available concerning the biogenesis of the phospholipid bilayer of the endoplasmic reticulum, particularly from the application of the structural cytochemical methods for the localization of acyltransferases, is reviewed. The observations are consistent with a model in which phospholipid is synthesized in situ at the site of membrane growth. Synthesis is asymmetric, with most enzymes located at the cytoplasmic side of the membrane, and controlled transmembrane movement of phospholipid results in an asymmetric bilayer.
内质网具备合成蛋白质和磷脂的酶机制，因此在自身生物合成以及其他细胞膜的生物合成过程中发挥着核心作用。本文综述了有关内质网磷脂双层生物合成的现有证据，尤其是应用结构细胞化学方法定位酰基转移酶所获得的证据。这些观察结果与一种模型相符，即磷脂在膜生长部位原位合成。合成过程是不对称的，大多数酶位于膜的细胞质一侧，磷脂的跨膜受控移动导致形成不对称的双层膜。
