Borisova O V, Rodionova N P, Nekliudova I V, Dolia V V, Atabekov I G
Mol Biol (Mosk). 1982 Sep-Oct;16(5):1041-50.
The purified preparation of cucumber virus 3 (CV3) (one of the member of tobamovirus group) contains, besides full-length (2.0 X 10(6)) genomic RNA, a short (0.24 X 10(6)) subgenomic RNA coding for coat protein in Xenopus laevis oocytes as well as in the cell-free protein synthesizing system from wheat embryos. The similarity of the polypeptide coded by short CV3 RNA in vitro to natural CV3 coat protein is proved by the similarity of their molecular weights, antigenic specificity and by the similarity of the peptide maps. Individual CV3 genomic RNA coded in vitro for polypeptide 130 X 10(3), and was incapable of coding for the coat protein. The results of translation of an artificial mixture of short and full-length RNAs suggest that it is the short RNA that is preferentially translated.
黄瓜病毒3（CV3）（烟草花叶病毒组的成员之一）的纯化制剂，除了全长（2.0×10⁶）基因组RNA外，在非洲爪蟾卵母细胞以及小麦胚无细胞蛋白质合成系统中还含有一种短的（0.24×10⁶）编码外壳蛋白的亚基因组RNA。体外由短CV3 RNA编码的多肽与天然CV3外壳蛋白在分子量、抗原特异性以及肽图的相似性方面得到了证实。体外编码的单个CV3基因组RNA产生了分子量为130×10³的多肽，并且不能编码外壳蛋白。短RNA和全长RNA人工混合物的翻译结果表明，优先被翻译的是短RNA。
