Basler A
Mutat Res. 1982 Sep;102(2):137-43. doi: 10.1016/0165-1218(82)90114-8.
A lymphocyte culture system was used to study cytogenetic effects in rats after they had been exposed to genotoxic agents in vivo. The sensitivity of the test system was demonstrated by using cyclophosphamide. The SCE frequencies in lymphocytes of cyclophosphamide-treated rats (13.3 mg/kg; i.p.) was increased about 4-fold compared with those in lymphocytes from untreated rats. Female rats of the strain Wistar II were exposed to cigarette smoke from 30 cigarettes per day in an inhalation chamber. Lymphocyte cultures were performed after an acute exposure and after treatment for 4 weeks. After both treatments, the investigations of chromosomes with regard to SCEs did not reveal any effect.
采用淋巴细胞培养系统研究大鼠体内暴露于遗传毒性剂后的细胞遗传学效应。通过使用环磷酰胺证明了该测试系统的敏感性。与未处理大鼠的淋巴细胞相比，经环磷酰胺处理的大鼠（13.3mg/kg；腹腔注射）淋巴细胞中的姐妹染色单体交换（SCE）频率增加了约4倍。Wistar II品系的雌性大鼠在吸入室中每天暴露于30支香烟的烟雾中。急性暴露后和处理4周后进行淋巴细胞培养。两种处理后，关于SCEs的染色体研究均未发现任何效应。
