Dunlop M, Court J M, Hobbs J B, Boulton T J
Pediatr Res. 1978 Sep;12(9):905-7. doi: 10.1203/00006450-197809000-00004.
In developing adipose tissue, cells of size less than 25 micron may make a major contribution to adipose tissue cell populations. This study reports the separation, identification, and sizing of infant and foetal adipose tissue small cells. Subcutaneous adipose tissue was taken from the anterior abdominal wall of children aged 2 months and 22 months. Subcutaneous tissue was also obtained from a paraumbilical site in 4 fetuses of gestational age 16 weeks. Tissue samples were fixed in buffered osmium tetroxide solution containing collidine. Aliquots of cell preparations suspended in saline on microscope slides were viewed and separated using a dissecting microscope. A diameter distribution of particle size was obtained using at least 200 cells for each preparation. There was a substantial number of cells that were smaller than 20 micron. Their modal cell size was remarkably constant in fetuses and in 2-month-old and 22-month-old infants. Ultrastructural studies show that the tissue is composed of a relatively uniform population of cells within sparsely collagenous connective tissue. Although some of the cells show no intracytoplasmic aggregates of lipid material, others show variable amounts of lipid material which can occupy a very large part of the cell volume.
在发育中的脂肪组织中，直径小于25微米的细胞可能对脂肪组织细胞群体有重要贡献。本研究报告了婴儿和胎儿脂肪组织小细胞的分离、鉴定及大小测定。从2个月和22个月大儿童的前腹壁获取皮下脂肪组织。还从16周胎龄的4例胎儿的脐旁部位获取皮下组织。将组织样本固定于含有可力丁的缓冲四氧化锇溶液中。将悬浮于盐水中的细胞制剂等分试样置于载玻片上，用解剖显微镜观察并分离。每种制剂至少使用200个细胞获得粒径分布。有大量细胞小于20微米。其模态细胞大小在胎儿以及2个月和22个月大的婴儿中非常恒定。超微结构研究表明，该组织由稀疏胶原结缔组织内相对均匀的细胞群体组成。尽管一些细胞未显示胞浆内脂质物质聚集，但其他细胞显示出数量可变的脂质物质，这些脂质物质可占据细胞体积的很大一部分。
