在改良海水介质中加热氨基酸产生的高分子量可溶性聚合物的形成及催化活性

Formation and catalytic activity of high molecular weight soluble polymers produced by heating amino acids in a modified sea medium.

作者信息

Okihana H

出版信息

Orig Life. 1982 Jun;12(2):153-63. doi: 10.1007/BF00927142.

Abstract

Eighteen protein amino acids with milk casein composition were heated in a modified sea medium. Marigranules were formed in the precipitates and soluble polymers were formed in the supernatant. Time course of the reaction (ultraviolet spectra, the concentration of metal ions, and the concentration of amino acids in the supernatant) were measured. The time course of the formation of the soluble polymers was also studied by Bio-Gel P-2 column. High molecular weight soluble polymers (HMWSP) were separated from low molecular weight ones by dialysis. It was shown that these polymers catalyzed the dehydrogenation of NADH. These polymers also catalyzed the coupled reaction between dehydrogenation of NADH and reduction of resazurin. This coupled reaction was accelerated by the light.

摘要

将18种具有乳酪蛋白组成的蛋白质氨基酸在改良的海水培养基中加热。沉淀物中形成了微球粒，上清液中形成了可溶性聚合物。测定了反应的时间进程（紫外光谱、金属离子浓度和上清液中氨基酸浓度）。还通过Bio-Gel P-2柱研究了可溶性聚合物形成的时间进程。通过透析将高分子量可溶性聚合物（HMWSP）与低分子量聚合物分离。结果表明，这些聚合物催化了NADH的脱氢反应。这些聚合物还催化了NADH脱氢与刃天青还原之间的偶联反应。光照加速了这种偶联反应。