Hughes W T, Koblin B A, Rosenstein B J
Pediatr Res. 1982 Oct;16(10):874-6. doi: 10.1203/00006450-198210000-00014.
The activity of lysozyme in saliva and serum was determined in 51 patients with cystic fibrosis. Measurements were made on two occasions at least 1 month apart and compared to those of 25 normal healthy individuals of the same ages, sex, and race. The mean serum lysozyme activity of normal individuals was 5.8 micrograms/ml (S.E. = 0.4), whereas that of cystic fibrosis patients was 10.8 micrograms/ml (S.E. = 0.5). The difference is significant (P less than 0.05). Initial mean values compared to those of repeated samples from the cystic fibrosis group were similar, whereas individual fluctuations occurred between test periods. The mean lysozyme activity of the saliva sample of normal individuals was 63.5 micrograms/ml (S.E. = 9.3) and the mean value from cystic fibrosis patients was 82.7 (S.E. = 6.9). This difference was not significant (P greater than 0.1). Mean values from specimens obtained a month or longer after the initial saliva samples were similar for the two episodes. There was no correlation between the serum and salivary values and the age, sex or race of the subjects, the Shwachman-Kulczycki scores, colonization with Pseudomonas aeruginosa, Staphylococcus aureus or Haemophilus influenzae or absolute white blood cell counts. In vitro studies failed to demonstrate bactericidal activity for mucoid and nonmucoid strains of P. aeruginosa or for S. aureus. Elevated lysozyme activity in cystic fibrosis may be related to either an increased granulocyte turnover because of chronic bacterial infection of the respiratory tract or to a basic defect in the lysosomal membrane allowing an increased release of the enzyme, or a combination of both.
对51例囊性纤维化患者的唾液和血清中的溶菌酶活性进行了测定。在至少相隔1个月的两个时间点进行测量，并与25名年龄、性别和种族相同的正常健康个体的测量结果进行比较。正常个体的血清溶菌酶平均活性为5.8微克/毫升（标准误=0.4），而囊性纤维化患者的血清溶菌酶平均活性为10.8微克/毫升（标准误=0.5）。差异具有显著性（P<0.05）。与囊性纤维化组重复样本的初始平均值相比相似，而在测试期间个体出现波动。正常个体唾液样本的溶菌酶平均活性为63.5微克/毫升（标准误=9.3），囊性纤维化患者的平均值为82.7（标准误=6.9）。这种差异不具有显著性（P>0.1）。在初始唾液样本采集1个月或更长时间后获得的标本的平均值在两次采样中相似。血清和唾液值与受试者的年龄、性别或种族、施瓦赫曼-库尔奇茨基评分、铜绿假单胞菌、金黄色葡萄球菌或流感嗜血杆菌定植情况或绝对白细胞计数之间无相关性。体外研究未能证明对铜绿假单胞菌的黏液型和非黏液型菌株或金黄色葡萄球菌具有杀菌活性。囊性纤维化患者溶菌酶活性升高可能与由于呼吸道慢性细菌感染导致的粒细胞周转率增加有关，或者与溶酶体膜的基本缺陷导致酶释放增加有关，或者是两者的结合。
