脱氧核糖环的13C核磁共振弛豫与构象灵活性
13C NMR relaxation and conformational flexibility of the deoxyribose ring.
作者信息
Levy G C, Craik D J, Chou Y C, London R E
出版信息
Nucleic Acids Res. 1982 Oct 11;10(19):6067-83. doi: 10.1093/nar/10.19.6067.
Abstract
13C T1's and NOE's have been measured for all protonated carbons of 2'-deoxy-D-ribose (2'-d-ribose), 2'-deoxyadenosine-5'-monophosphate (5'-dAMP), thymidine-3'-monophosphate (3'-TMP) and thymidine-5'-monophosphate (5'-TMP) in D2O solutions. In all of the deoxy sugars examined, NT1 values for C-2' are significantly larger than the values for the remaining carbons. This result is interpreted in terms of rapid puckering motion of C-2'. By contrast, NT1 values measured in ribose are found to be equal, within experimental error. The results are compared with analogous data obtained for the five membered pyrrolidine ring of proline and with results for DNA itself.
摘要
已在重水（D2O）溶液中测量了2'-脱氧-D-核糖（2'-d-核糖）、2'-脱氧腺苷-5'-单磷酸（5'-dAMP）、胸苷-3'-单磷酸（3'-TMP）和胸苷-5'-单磷酸（5'-TMP）所有质子化碳的13C T1值和核Overhauser效应（NOE）。在所有检测的脱氧糖中，C-2'的NT1值显著大于其余碳的NT1值。这一结果可根据C-2'的快速折叠运动来解释。相比之下，在核糖中测量的NT1值在实验误差范围内是相等的。将这些结果与脯氨酸五元吡咯烷环的类似数据以及DNA本身的结果进行了比较。
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